研究领域：纳米技术 生物医学工程 药学 伤口愈合

关键字：介孔聚多巴胺纳米颗粒 肽RL-QN15 皮肤伤口愈合 纳米复合材料 促愈活性 持续释放

背景：皮肤伤口愈合仍是重大的临床挑战，亟需开发促进修复的新干预策略。近期研究表明，多肽与纳米颗粒均可能成为治疗皮肤伤口的潜在疗法。方法：本研究制备了介孔聚多巴胺（MPDA）纳米颗粒，并将前期从两栖动物皮肤分泌物中鉴定获得、具有显著促愈合潜力的多肽RL-QN15成功负载于MPDA纳米颗粒表面；扫描电子显微镜与傅里叶变换红外光谱分析结果证实了负载成功。测定该纳米复合物对RL-QN15的包封率及缓释速率，并通过小鼠与猪全层皮肤缺损创面模型以及小鼠烧伤创面模型评估其促愈合效能。结果：结果显示，相较于游离RL-QN15，MPDA/RL-QN15纳米复合物通过RL-QN15的缓释作用，在小鼠全层皮肤缺损创面及烧伤创面模型中的促愈合效能最高提升达50倍；此外，MPDA负载亦显著增强RL-QN15在猪全层皮肤缺损创面模型中的促愈合活性。组织病理学分析结果进一步证实MPDA/RL-QN15纳米复合物促愈合效能的显著提升。结论：据我们所知，本研究首次报道MPDA纳米颗粒负载可显著增强多肽的促愈合效能，凸显MPDA纳米颗粒递送多肽RL-QN15在皮肤伤口治疗中的广阔应用前景。Background Skin wound healing remains a considerable clinical challenge, thus stressing the urgent need for the development of new interventions to promote repair. Recent researches indicate that both peptides and nanoparticles may be potential therapies for the treatment of skin wounds.Methods In the current study, the mesoporous polydopamine (MPDA) nanoparticles were prepared and the peptide RL-QN15 that was previously identified from amphibian skin secretions and exhibited significant potential as a novel prohealing agent was successfully loaded onto the MPDA nanoparticles, which was confirmed by results of analysis of scanning electron microscopy and fourier transform infrared spectroscopy. The encapsulation efficiency and sustained release rate of RL-QN15 from the nanocomposites were determined. The prohealing potency of nanocomposites were evaluated by full-thickness injured wounds in both mice and swine and burn wounds in mice.Results Our results indicated that, compared with RL-QN15 alone, the prohealing potency of nanocomposites of MPDA and RL-QN15 in the full-thickness injured wounds and burn wounds in mice was increased by up to 50 times through the slow release of RL-QN15. Moreover, the load on the MPDA obviously increased the prohealing activities of RL-QN15 in full-thickness injured wounds in swine. In addition, the obvious increase in the prohealing potency of nanocomposites of MPDA and RL-QN15 was also proved by the results from histological analysis.Conclusion sBased on our knowledge, this is the first research to report that the load of MPDA nanoparticles could significantly increase the prohealing potency of peptide and hence highlighted the promising potential of MPDA nanoparticles-carrying peptide RL-QN15 for skin wound therapy.Graphic abstract