Rab7 Pull-Down Activation Assay Kit

Cat. # TD-82501

Background

Small GTPases are a super-family of cellular signaling regulators. The small Ras-like GTPase Rab7 is an evolutionary conserved protein that originally gained interest because of its capacity to revert the morphological phenotype of Ras-transformed fibroblasts. Rab7 is regulated by a large number of stimuli that include growth factors and cytokines, but also physical force and osmotic stress. Rab7 was shown to regulate multiple basic cellular processes. The best studied aspect of Rab7 function in endothelial cells involved its role in regulation of cell-cell junction formation and remodeling.

Rab7 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab7-GTP, but not Rab7-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

The anti-Rab7-GTP monoclonal antibody can also be used to monitor the activation of Rab7 in cells and in tissues by immunohistochemistry.

Assay Principle

The Rab7 Activation Assay Kit uses configuration-specific anti-Rab7-GTP Mouse monoclonal antibody to measure Rab7-GTP levels in cell extracts or in vitro GTPγS loading Rab7 activation assays. Anti-Rab7-GTP mouse monoclonal antibody is first incubated with cell lysates containing Rab7-GTP. Next, the GTP-bound Rab7 is pulled down by protein A/G agarose. Finally, the precipitated Rab7-GTP is detected through immunoblot analysis using Anti-Rab7 Rabbit Polyclonal Antibody.