Rab11 Pull-Down Activation Assay Kit

Cat. # TD-83201

Background

Small GTPases are a super-family of cellular signaling regulators. Rab11 has been shown to control traffic through the recycling endosome.

The Rab11 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab11-GTP, but not Rab11-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

Currently there is no direct assay to measure the activation of Rab11 GTPases.

Assay Principle

The Rab11 Activation Assay Kit uses configuration-specific anti-Rab11-GTP Mouse monoclonal antibody to measure Rab11-GTP levels in cell extracts or in vitro GTPγS loading Rab11 activation assays. Anti-Rab11-GTP mouse monoclonal antibody is first incubated with cell lysates containing Rab11-GTP. Next, the GTP-bound Rab11 is pulled down by protein A/G agarose. Finally, the precipitated Rab11-GTP is detected through immunoblot analysis using Anti-Rab11 Rabbit Polyclonal Antibody.