Rab3A Pull-Down Activation Assay Kit

Cat. # TD-82601

Background

Rab3A is a member of the Rab-family GTPases and is specifically targeted to synaptic vesicles Currently there is no direct assay to measure the activation of Rab3 GTPases.

The Rab3A Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab3-GTP, but not Rab3-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

Currently there is no direct assay to measure the activation of Rab3 GTPases.

Assay Principle

The Rab3A Activation Assay Kit uses configuration-specific anti-Rab3-GTP Mouse monoclonal antibody to measure Rab3-GTP levels in cell extracts or in vitro GTPγS loading Rab3 activation assays. Anti-Rab3-GTP mouse monoclonal antibody is first incubated with cell lysates containing Rab3-GTP. Next, the GTP-bound Rab3 is pulled down by protein A/G agarose. Finally, the precipitated Rab3-GTP is detected through immunoblot analysis using Anti-Rab3A Rabbit Polyclonal Antibody.