Arl3 Pull-Down Activation Assay Kit

Cat. # TD-83001

Background

Arl3 (Are-like 3) is an ADP-ribosylation factor (AR) family protein that differs from most are family members in the N-terminal extension. Nucleotide exchange of Arl3 is rapid and independent of lipids and detergents. Upon binding of GDT/GTP, Arl3 interacts with and regulates activities of series effector proteins, such as human retinal gene 4 (HRG4), δ-subunit of the cGMP phosphodiester (PDEδ) and binder of Arl2 (BART). Arl3 also binds microtubules in a regulated manner to alter specific aspects of cytokinesis via interactions with retinitis pigmentosa 2 (RP2). It has been proposed that RP2 functions in concert with Arl3 to link the cell membrane and the cytoskeleton in photoreceptors as part of the cell signaling or vesicular transport machinery.

Assay Principle

The Arl3 Activation Assay Kit uses configuration-specific anti-Arl3-GTP Mouse monoclonal antibody to measure Arl3-GTP levels in cell extracts or in vitro GTPγS loading Arl3 activation assays. Anti-Arl3-GTP mouse monoclonal antibody is first incubated with cell lysates containing Arl3-GTP. Next, the GTP-bound Arl3 is pulled down by protein A/G agarose. Finally, the precipitated Arl3-GTP is detected through immunoblot analysis using anti-Arl3 mouse monoclonal antibody.