Rab11,Rab11-GTP

Activation Assay Kit

Configuration-specific monoclonal antibody that recognizes Rab11-GTP, but not Rab11-GDP The only available Rab11 activation assay kit directly measures the level of Rab11-GTP Providing shorter experimental time Could be used to monitor the activation of Rab11 in cells and in tissues by immunohistochemistr

Tanda Biosciences Rab11 Activation Assay Kit bases on the configuration-specific anti- Rab11-GTP monoclonal antibody to measure the active Rab11-GTP levels, either from cell extracts or from in vitro GTPγS loading Rab11 activation assays. Briefly, anti-active Rab11 mouse monoclonal antibody will be incubated with cell lysates containing Rab11-GTP. The bound active Rab11 will then be pulled down by protein A/G agarose. The precipitated active Rab11 will be detected by immunoblot analysis using an anti-Rab11 rabbit polyclonal antibody.

Small GTPases are a super-family of cellular signaling regulators. Rab11 has been shown to control traffic through the recycling endosome. Currently there is no direct assay to measure the activation of Rab GTPases. Tanda Biosciences Rab11 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab11-GTP, but not Rab11-GDP, and a Rab11 specific rabbit polyclonal antibody. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility. This anti-Rab11-GTP monoclonal antibody can also be used to monitor the activation of Rab11 in cells and in tissues by immunohistochemistry. Tanda Biosciences Rab11 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab11-GTP, but not Rab11-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

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