Product Name | Anti-6X His Tag antibody |
Cat.# | TD-AB0013 |
Package | 25 μl/100 μl/200 μl |
Storage | PBS with 0.1% sodium azide and 50% glycerol, pH 7.2 |
Product Imformation | |
Description | Anti-6×His Tag mouse monoclonal antibody |
Applications | WB, IF, IP |
Immunogen | Synthetic peptide conjugated with KLH |
Reactivity | Fusion proteins containing the His Tag |
Host species | Mouse |
Ig class | IgG1 |
Purity | Protein A/G Affinity Purification |
Target information | |
Symbol | 6X His tag |
Full name | HHHHHH |
Target Background | |
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. | |
Western blotting | |
Predicted band size | 50 kDa |
Positive control | Fusion proteins containing the His Tag |
Recommended dilution | 1000-5000 |
Product Image | |
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