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Anti Active RhoA Mouse Monoclonal Antibody
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TD-26904
- Catalog： TD-26904
- Clonality： 单克隆
- Application： IP, IHC
- Synonyms： Active RhoA-GTP
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规格：
30ul
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价格：
¥4800
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RhoA-GTP
| Cat. #: TD-26904 | |
| Size: 30 µL | |
| Gene Symbol: RHOA | |
| Description: Anti-RhoA-GTP Mouse Monoclonal Antibody | |
| Background: Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction. | |
| Immunogen: Recombinant full length protein of active RhoA | |
| Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) | |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 | |
| Concentration: 1 mg/ml | |
| Host Species: Mouse | |
| Format: Liquid | |
| Clonality: Monoclonal | |
| Isotype: IgG2b | |
| Purity: Purified from ascites | |
| Preservative: No | |
| Constituents: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol | |
| Species Reactivity: Anti-RhoA monoclonal antibody recognizes active RhoA from vertebrates. | |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing | |
Immunoprecipitation/Western Blot:  |
IP/WB Analysis of Active RhoA Protein. Purified recombinant RhoA proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-RhoA-GTP monoclonal antibody (Cat. # TD-26904). After SDS/PAGE, the membrane filter was probed with anti-RhoA mouse monoclonal antibody. |