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Anti Active Rap1 Mouse Monoclonal Antibody
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TD-26912
- Catalog： TD-26912
- Clonality： 单克隆
- Application： IP, IHC, IF
- Synonyms： Active Rap1-GTP
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规格：
30ul
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价格：
¥4800
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Rap1-GTP
| Cat. #: TD-26912 | |
| Size: 30 µL | |
| Gene Symbol: RAP1 | |
| Description: Anti-Rap1-GTP Mouse Monoclonal Antibody | |
| Background: Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway, Raf-1 and B-Raf. Rap1 regulates cell adhesion mechanisms through effector molecules such as the integrins and cadherins. | |
| Immunogen: Recombinant full length protein of active Rap1. | |
| Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) | |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 | |
| Concentration: 1 mg/ml | |
| Host Species: Mouse | |
| Format: Liquid | |
| Clonality: Monoclonal | |
| Isotype: IgG | |
| Purity: Purified from ascites | |
| Preservative: No | |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | |
| Species Reactivity: Anti-Rap1-GTP monoclonal antibody recognizes active Rap1 of vertebrates. | |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing | |
|
Immunoprecipitation/Western blot: |
Rap1 Activation Assay.Purified Rap1 proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rap1-GTP monoclonal antibody (Cat. No. TD-26912). Immunoblot was with an anti-Rap1 polyclonal antibody (Cat. No. ). |