Catalog Number: TD-26924 | |
Gene Symbol: Arl1 | |
Description: Anti active Arl1 Mouse Monoclonal Antibody | |
Background: Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Golgi-localized proteins golgin-97 and golgin-245 have been shown as Arl1 effectors. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. In Drosophila zygotes, mutation of Arl1 is lethal. | |
Immunogen: Recombinant Arl1 protein (active form) | |
Applications: IP, IHC, IF | |
Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP 、1:50~100 dilution for IHC, IF | |
Concentration: 1 mg/ml | |
Host: mouse | |
Format: Liquid | |
Clonality: Monoclonal | |
Isotype: IgG | |
Purity: Purified from ascites | |
Storage buffer: | |
Preservative: no | |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | |
Species Reactivity: Arl1 antibody recognizes active Arl1-GTP from vertebrates. | |
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles | |
Immunoprecipitation/Western blot: | |
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Arl1 Activation Assay. Purified GST-tagged Arl1 proteins were immunoprecipitated with the anti active Arl1 monoclonal antibody (Cat. # TD-26924) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti Arl1 monoclonal antibody. Arl1 protein input control is shown in the bottom panel. |