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Anti Active Sar1 Mouse Monoclonal Antibody
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TD-26916
- Catalog： TD-26916
- Clonality： 单克隆
- Application： IP, IHC, IF
- Synonyms： Active Sar1-GTP
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规格：
30ul
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价格：
¥4800
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Sar1-GTP
| Cat. #: TD-26916 | |
| Size: 30 µL | |
| Gene Symbol: SAR1 | |
| Description: Anti-Sar1-GTP Mouse Monoclonal Antibody | |
| Background: Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER. | |
| Immunogen: Recombinant full length protein of active Sar1a. | |
| Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) | |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins、IHC, IF: 1:50-1:250 | |
| Concentration: 1 mg/ml | |
| Host Species: Mouse | |
| Format: Liquid | |
| Clonality: Monoclonal | |
| Isotype: IgG | |
| Purity: Purified from ascites | |
| Preservative: No | |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | |
| Species Reactivity: Anti-active Sar1 antibody recognizes active Sar1 of vertebrates. | |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing | |
|
Immunoprecipitation/Western blot:
|
Sar1 Activation Assay. Purified Sar1 proteins were loaded with GDP (lanes 2 and 3) or GTPγS (lanes 4 and 5). Immunoprecipitation was done with the anti-active Sar1 monoclonal antibody (Cat. No. 26916). Immunoblot was with an anti-Sar1 rabbit polyclonal antibody . |