Cat. #: TD-26910 | |
Size: 30 µL | |
Gene Symbol: RHEB | |
Description: Anti-Rheb-GTP Monoclonal Antibody | |
Background: Small GTPases are a super-family of cellular signaling regulators. Rheb is a member of the Ras-superfamily GTPases. Rheb has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related (ATR). | |
Immunogen: Recombinant full-length protein of active Rheb. | |
Applications: IP, IHC, IF | |
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 | |
Concentration: 1 mg/ml | |
Host Species: Mouse | |
Format: Liquid | |
Clonality: Monoclonal | |
Isotype: IgM | |
Purity: Purified from ascites | |
Preservative: No | |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | |
Species Reactivity: Anti-Rheb-GTP monoclonal antibody recognizes active Rheb of vertebrates. | |
Storage Conditions: Store at -20°C. Avoid freeze/thaw cycles | |
Immunoprecipitation/Western blot:
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Rheb activation assay. Purified Rheb proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rheb-GTP monoclonal antibody (Cat. No. TD-26910). Immunoblot was with an anti-Rheb rabbit polyclonal antibody . |