Cat. #: TD-26906 | |
Size: | |
Gene Symbol: Gnas | |
Description: Anti-Gαs-GTP Mouse Monoclonal Antibody | |
Background: Heterotrimeric G proteins are critical cellular signal transducers. Gαs represents one sub-family of G proteins that could mediate the stimulation of adenylyl cyclases. | |
Immunogen: Recombinant full length protein of active Gαs | |
Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) | |
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 | |
Concentration: 1 mg/ml | |
Host Species: | |
Format: Liquid | |
Clonality: Monoclonal | |
Isotype: IgG | |
Purity: Purified from ascites | |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | |
Species Reactivity: Anti-active Gαs antibody recognizes active Gαs of vertebrates | |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing | |
Immunoprecipitation/Western blot:
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Gαs Activation Assay. Purified Gαs proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti Gαs-GTP monoclonal antibody (Cat. No. TD-26906). Immunoblot was with an anti- Gαs monoclonal antibody . |