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Anti Active Gαs Mouse Monoclonal Antibody
All
IP
  • CatalogTD-26906
  • Clonality单克隆
  • Application IP, IHC
  • SynonymsActive Gαs-GTP
  • 规格
    30ul
  • 价格
    ¥4800
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s-GTP


Cat. #:   TD-26906
Size:
Gene Symbol:   Gnas
Description:   Anti-Gαs-GTP Mouse Monoclonal Antibody
Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαrepresents one sub-family of G proteins that could mediate the stimulation of adenylyl cyclases.
Immunogen:   Recombinant full length protein of active Gαs
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:   IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:   Anti-active Gαs antibody recognizes active Gαs of vertebrates
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation/Western blot:  

 

 

s Activation Assay. Purified Gαs proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti Gαs-GTP monoclonal antibody (Cat. No. TD-26906). Immunoblot was with an anti- Gαmonoclonal antibody .